Vasculata 2024 Sessions
scRNA sequencing, nuclear sequencing – platforms, interpretation, validation I
Monday Workshop (1:30pm)
Speakers
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Amy Tian - Workshop Leader
Summary
This workshop will provide a comprehensive introduction to the computational analysis of single-cell RNA sequencing (scRNA-seq) data, including current scRNA-seq technologies and best practices for analyzing gene expression at the single-cell level. The focus will be on practical applications and interpretation of scRNA-seq data. By the end of this workshop, students will have a solid understanding of the computational tools and techniques used in scRNA-seq, enabling them to critically assess and apply these technologies in their future careers.
Multiplex spatial techniques I
Monday Workshop (1:30pm)
Summary
The talk will review different techniques for multiplex spatial localization of mRNA and protein in tissue, including multiplex antibody-based imaging, single molecule RNA fluorescent in situ hybridization (smFISH), hybridization chain reaction (HCR) FISH, proximity ligation techniques, merFISH, and potentially dissection-based methods, and sequencing based methods. There will be an additional effort to cover some practical topics with regard to tissue preparation, imaging, and data processing.
2D Versus 3D Imaging I
Monday Workshop (1:30pm)
Speakers
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Helen Turton - Workshop Leader
Summary
This session is in collaboration with Leica (confocal microscopy) and Imaris (imaging software).
The session will provide an introduction to 3D-deep tissue imaging in vascular biology, delivered in the form of a 30-40 minute lecture with a short Q & A session. This session aims to explain the principles and the practical workflow of 3D-deep tissue imaging. There will be a heavy focus on protocol explanation, highlighting the specialist technical requirements of tissue clearing, confocal microscopy by Leica, and Imaris Software to render 3D projections, visualize, and analyze images.
Multiplex spatial techniques II
Monday Workshop (2:30pm)
Summary
The talk will review different techniques for multiplex spatial localization of mRNA and protein in tissue, including multiplex antibody-based imaging, single molecule RNA fluorescent in situ hybridization (smFISH), hybridization chain reaction (HCR) FISH, proximity ligation techniques, merFISH, and potentially dissection-based methods, and sequencing based methods. There will be an additional effort to cover some practical topics with regard to tissue preparation, imaging, and data processing.
scRNA Sequencing, Nuclear sequencing – Platforms, Interpretation, and Validation II
Monday Workshop (2:30pm)
Summary
This workshop will provide a comprehensive introduction to the computational analysis of single-cell RNA sequencing (scRNA-seq) data, including current scRNA-seq technologies and best practices for analyzing gene expression at the single-cell level. The focus will be on practical applications and interpretation of scRNA-seq data. By the end of this workshop, students will have a solid understanding of the computational tools and techniques used in scRNA-seq, enabling them to critically assess and apply these technologies in their future careers.
2D Versus 3D Imaging II
Monday Workshop (2:30pm)
Speakers
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Helena Turton - Workshop Leader
Summary
This session is in collaboration with Leica (confocal microscopy) and Imaris (imaging software).
The session will provide an introduction to 3D-deep tissue imaging in vascular biology, delivered in the form of a 30-40 minute lecture with a short Q & A session. This session aims to explain the principles and the practical workflow of 3D-deep tissue imaging. There will be a heavy focus on protocol explanation, highlighting the specialist technical requirements of tissue clearing, confocal microscopy by Leica, and Imaris Software to render 3D projections, visualize, and analyze images.
2D Versus 3D Imaging III
Monday Workshop (4:00pm)
Speakers
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Helena Turton - Workshop Leader
Summary
This session is in collaboration with Leica (confocal microscopy) and Imaris (imaging software).
The session will provide an introduction to 3D-deep tissue imaging in vascular biology, delivered in the form of a 30-40 minute lecture with a short Q & A session. This session aims to explain the principles and the practical workflow of 3D-deep tissue imaging. There will be a heavy focus on protocol explanation, highlighting the specialist technical requirements of tissue clearing, confocal microscopy by Leica, and Imaris Software to render 3D projections, visualize, and analyze images.
scRNA sequencing, nuclear sequencing – platforms, interpretation, validation III
Monday Workshop (4:00pm)
Summary
This workshop will provide a comprehensive introduction to the computational analysis of single-cell RNA sequencing (scRNA-seq) data, including current scRNA-seq technologies and best practices for analyzing gene expression at the single-cell level. The focus will be on practical applications and interpretation of scRNA-seq data. By the end of this workshop, students will have a solid understanding of the computational tools and techniques used in scRNA-seq, enabling them to critically assess and apply these technologies in their future careers.
Multiplex spatial techniques III
Monday Workshop (4:00pm)
Summary
The talk will review different techniques for multiplex spatial localization of mRNA and protein in tissue, including multiplex antibody-based imaging, single molecule RNA fluorescent in situ hybridization (smFISH), hybridization chain reaction (HCR) FISH, proximity ligation techniques, merFISH, and potentially dissection-based methods, and sequencing based methods. There will be an additional effort to cover some practical topics with regard to tissue preparation, imaging, and data processing.
Grant writing workshop
Tuesday Workshop
Speakers
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Crystal Botham
Summary
Looking at specific aims, and how to write a powerful abstract
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